One of the missions of the Wisconsin Alzheimer's Disease Research Center (ADRC) is to help further scientific research in Alzheimer's disease and related disorders. To that effect, we make our data available to investigators who are approved through our electronic Resource Request Application.
Visit our Apply for Resources page for application information.
In addition, the Wisconsin ADRC offers a variety of researcher services available through the respective service leaders.
Explore the drop-down lists below to learn more about Wisconsin ADRC researcher resources and services, or download a PDF summarizing our resources and services for researchers.
Human Subjects Data
Interview and Questionnaire Data
Available from all participants enrolled in the Clinical Core. Our longitudinal data includes variables collected as part of the Uniform Data Sets (UDS) from the National Alzheimer’s Coordinating Center's (NACC), as well as center-specific variables that may be unique to the Wisconsin ADRC. See Table 1 for a complete list of UDS and Wisconsin ADRC forms. A detailed description of the UDS forms is available on the NACC website. NACC also maintains an extensive Researcher Data Dictionary with information about current and previous UDS form versions. The Wisconsin ADRC Data Dictionary contains a list of center specific variables.
Cognitive and Psychological Variables
Cognitive testing includes the NACC battery, the Rey AVLT, and the NIH Toolbox. The cognitive battery is administered every year to controls over 65 years old and to participants with a cognitive impairment; younger controls ages 45 to 65 are scheduled for research visits every other year. Longitudinal data is available, with some participants having completed more than 10 visits with cognitive testing. Starting in August 2020, our center also started collecting cognitive data over the phone. For more information about the current NACC battery, please view Form C2: Neuropsychological Battery Scores and Form C2T:Neuropsychological Battery Scores for T-cog on the NACC website.
In addition to completing cognitive testing, Wisconsin ADRC participants donate blood, cerebrospinal fluid (CSF), and provide neuroimaging data. About 75% of all ADRC Clinical Core participates have completed at least one MRI and close to 45% have completed one or more lumbar punctures. Additionally, participants have the option to undergo PET scans.
- CSF has been historically analyzed in batches, using a crosswalk to convert values and work around lot-to-lot variability. More recently, our center has entered into a collaboration with Roche Diagnostics and has acquired an Elecsys® Immunoassay Analyzer in order to be able to run CSF samples in house using an automated process.
- Blood-based biomarkers are likely to change the way clinicians establish a clinical diagnosis of Alzheimer’s disease. Our center collects whole blood, plasma, and serum from all Clinical Core participants. DNA from whole blood is analyzed after the first visit to determine each participants’ Apolipoprotein E (ApoE) genotype. We also collaborate with other institutions to determine levels of amyloid and tau proteins based on plasma samples.
- Neuroimaging Data are collected from all eligible participants. Images are collected according to the Standardized Centralized Alzheimer’s & Related Dementias Neuroimaging (SCAN) criteria. Brain imaging is performed soon after a Clinical Core visit (Baseline or annual follow-up), and can be linked to variables collected at Core Visits. MRI scans are obtained prior to lumbar puncture (another, separate and optional procedure). Neuroimaging data collected from Clinical Core participants include:
- Anatomical MRI scans: These are standard scanner sequences weighted for identifying and quantifying brain tissue health and volume. These include T1-weighted, T2 weighted, T2* weighted and diffusion weighted scans.
- Blood flow MRI scans: Scans weighted for cerebral blood flow are obtained. These include multi-delay arterial spin labeling and 4-flow phase contrast methods that measure intrinsic flow-related signal. No IV-contrast is administered.
- [11C]PiB PET scans: Scans for detecting amyloid burden. Images are obtained from 0-70 minutes or 50-70 minutes post-injection of a target dose of 15 mCi of [11C]PiB.
- [18F]MK6240 PET scans: Scans for detecting tau burden. Images are obtained from 70-110 minutes post-injection of 5-10 mCi of [18F]MK6240.
Laboratory and Clinical Data
Our center performs laboratory tests and collects vital signs at each in-person initial and follow-up visit. A list of tests in available in Table 1.
Neuropathological data set
This set contains autopsy data and a standardized evaluation form provided by NACC. The Researchers Data Dictionary is the primary resource for investigators interested in analyzing neuropathology data.
Wisconsin ADRC Participants and Caregivers
Clinical Core study participants
The Clinical Core observational study provides investigators with access to a diverse cohort of study participants. Participants in the Clinical Core fall within the following cohorts:
- Diagnosis of dementia due to Alzheimer’s disease
- Diagnosis of mild cognitive impairment
- Adults 45-65 with or without a parental history of Alzheimer’s disease
Approximate racial breakdown
- 76% Caucasian
- 18% African American
- 6% Native American
Participants in the Clinical Core Registry complete comprehensive clinical evaluations and cognitive assessments, either on an annual or biannual basis. The evaluations and assessments generate high-quality, standardized clinical, cognitive, biomarker samples, and neuroimaging data.
- Annual and biannual visits
- Fasting blood draw
- Physical exam
- Cognitive testing
- Biomarker visits
- MRI scan
- CSF collection
- PET scan
Wisconsin ADRC Research Recruitment Registry
The Wisconsin ADRC Research Recruitment Registry is made up of a group of people 18 years and older who are interested in joining research studies related to Alzheimer’s disease. When someone joins the recruitment registry we ask them demographic and some health-related questions that can be found in our first contact form. (link FCF). Some participants are better characterized than others if they have participated in other research studies within our center.
Dementia Care Research Core Recruitment Registry
The Dementia Care Research Core Recruitment Registry was established as a resource for future care-focused clinical studies, made up of caregiver and participant dyads. The registry provides investigators with the ability to recruit
- Caregivers of patients with diagnosed Alzheimer’s disease, dementia or memory problems
- Participant with diagnosis of Alzheimer’s disease, dementia or memory problems
- People 18 years or older
Participant with diagnosis and their caregiver agree to be contacted for:
- New research studies
- A baseline survey phone call and annual survey phone calls
Stored Biological Samples
Banked tissues collected ante-mortem
Serum, plasma, whole blood, and DNA are collected in a fasting state from all Clinical Core participants and banked. Samples are stored in 0.5–1ml aliquots at -80 degrees. Cerebrospinal Fluid (CSF) from individuals eligible and willing to complete lumbar puncture is also available.
Banked tissue collected post-mortem
The Wisconsin Brain Donor Program Tissue Catalog contains over 500 post-mortem biospecimen donations, including frozen and fixed brain tissues, frozen CSF, frozen whole blood, and formalin-fixed-paraffin-embedded tissue blocks and slides. While the brain bank is primarily focused on AD diagnoses, the donors span the gamut of neurodegenerative diseases: ADNC, LBD, ALS, FTLD, and CADASIL are just a few examples of the varied neuropathologic diagnoses that are available. Some of our brain donors were also enrolled in the Wisconsin ADRC Clinical Core or the Wisconsin Registry for Alzheimer's Prevention (WRAP) study, and additional research data may be available. For donors not enrolled in these studies, only limited demographic data is provided. Download the current Tissue Catalog.
The Genotyping Service can consult with investigators on selecting genotype analyses for particular research projects.
The Genotyping Service can customize genotyping to any genetic loci, and is experienced in performing genotying for the determination of single nucleotide polymorphisms (SNPs) and genetic repeats in several disease-related genes, including:
- Apolipoprotein E (exonic and promoter regions)
- Clusterin (apolipoprotein J)
- Androgen receptor
For more information contact, Craig Atwood, PhD.
Data Collection, Statistical and Epidemiological Services
Investigators can seek consultation and/or collaboration from the Data Management and Biostatistics Core’s (Core C) Data Collection, Statistical and Epidemiological Service regarding study initiation and data analysis.
Study initiation services:
- Study design
- Sample size estimates and power calculation
- Data collection planning
Data analysis services:
- Clinical trials
- Observational studies
Requests for statistical collaboration via investigators in Core C should be directed to both Rick Chappell, PhD, and Yue Ma, PhD. Please give your name, the name of the project's PI, its title and a brief summary, whether it currently has any grant support, whether one of the goals of collaboration is to apply for a grant, and your contact information.
Neuropsychological Evaluation Service
For studies involving collection of cognitive data, the Clinical Core’s (Core B) Neuropsychology Service can provide the following support:
Study consultation services:
- Designing a cognitive assessment battery
- Summarizing and interpreting neuropsychological data
- Data collection by Wisconsin ADRC staff
- Training research staff
For more information, contact Lindsay Clark, PhD.
Cerebrospinal Fluid (CSF) Collection Service
The Wisconsin ADRC CSF Collection Service follows a state-of-the-art protocol for specimen collection. This protocol was developed through collaborations with leading scientists in the field of CSF biomarker research. The Clinical Core’s (Core B) CSF Collection Service can assist investigators in several ways:
Study consultation services:
- Designing collection and storage methods
- Selecting CSF biomarker outcomes
- Sample collection by Wisconsin ADRC faculty
- Training study personnel on collection procedures
For more information, contact Cynthia Carlsson, MD, MS.
Investigators examining neuroimaging outcomes in patients with AD and MCI, as well as preclinical and at-risk populations, can obtain assistance from the Biomarker Core's (Core G) Neuroimaging Service:
- MRI protocol
- PET protocol
- Scan parameters
- Selecting most appropriate scans for study outcome
- Budgeting scan costs and personnel support
- FDA/IND and IRB approval and compliance monitoring
- Assist in MRI and PET protocol setup
- Train personnel in image transfer
Imaging training and guidance:
- Train personnel in specifics on consenting participants to imaging
- Train personnel in documentation required for MRI and PET
- Guidance about best practices for new imaging studies
- Quality control check
- Archiving images
For more information, contact Sterling Johnson, PhD.
Biomarker and Assay Service
The Biomarker and Assay Service can consult with investigators regarding sample collection, sample storage methods, and analyses or assays relevant to the research study.
The service has experience in performing assays in various biological samples including whole tissue (brain), whole blood, CSF, plasma, serum, saliva, and urine. Current assays include saliva, plasma, serum hormones (sex steroids and gonadotropins), whole tissue, plasma, CSF amyloid beta, tau, and tau phosphorylation.
For more information contact, Craig Atwood, PhD.
Care Research Consultation
The Care Research Core at the Wisconsin Alzheimer’s Disease Research Center offers researchers consultations and study design support. If you would like to learn more about care research resources, please complete the Dementia Care Research Core Consultation Form. A member of the Care Research Core will contact you about your request within three business days.